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For lysates preparation ( 31 ), cultures were centrifuged (10,000 g, 15 minutes), washed twice with potassium phosphate buffer (100 mM, pH 7.0), resuspended in borate buffer (50 mM, pH=8.4), and broken by mechanical lysis with glass beads in a tissue lyser (Qiagen, Courtaboeuf, France) 10 minutes, 50 Hz

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That was distressing, but not shocking

On the downside, vitamin B12 injections can be quite painful

Additionally, an acute toxicity test showed that 80% methanol avocado seed extract did not cause death or overt toxicity signs at any tested doses in mice, suggesting an LD 50 greater than 4,000 mg/kg (686)